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Irene Blanca-Reyes , María Torés-España , Victor Lechuga , María Teresa Llebrés , Fernando N. de la Torre , José A. Carreira , Concepción Avila , Francisco M. Cánovas , Vanessa Castro-Rodríguez , Dynamics of the Spanish fir transcriptome in nature: Metabolic pathways and gene networks involved in the response to climate stress Plant Stress (2025) , doi: https://doi.org/10.1016/j.stress.2025.101009
Sunal E, Castro-Rodriguez V*, Sadoine M*. Fluorophore-based Genetically Encoded Biosensors for Ratiometric Fluorescence Imaging in Microbes. J Vis Exp. 2025 Aug 1;(222). , doi: 10.3791/68339
Concepción Ávila, María Teresa Llebrés, Francisco M Cánovas, Vanessa Castro-Rodríguez, Arginine, a key amino acid for nitrogen nutrition and metabolism of forest trees Journal of Experimental Botany , 2025;, eraf260, https://doi.org/10.1093/jxb/eraf260
De La Torre, F., Medina-Morales, B., Blanca-Reyes, I., Pascual, M. B., Ávila, C., Cánovas, F. M., & Castro-Rodríguez, V. (2024). Properties and Functional Analysis of Two Chorismate Mutases from Maritime Pine Cells , 13 (11), 929. https://doi.org/10.3390/cells13110929
Blanca-Reyes, I., Lechuga, V., Llebrés, M. T., Carreira, J. A., Ávila, C., Cánovas, F. M., & Castro-Rodríguez, V. (2024). Under Stress: Searching for Genes Involved in the Response of Abies pinsapo Boiss to Climate Change. International Journal of Molecular Sciences , 25 (9), 4820. https://doi.org/10.3390/ijms25094820
Valderrama-Martín, J. M., Ortigosa, F., Cantón, F. R., Ávila, C., Cañas, R. A., and Cánovas, F. M. (2023). Emerging insights into nitrogen assimilation in gymnosperms. Trees , 1-14.
Valderrama-Martín JM, Ortigosa F, Aledo JC, Ávila C, Cánovas FM, Cañas RA. Pine has two glutamine synthetase paralogs, GS1b.1 and GS1b.2, exhibiting distinct biochemical properties. doi: 10.1111/tpj.16113
Sadoine M, De Michele R, Župunski M, Grossman G, Castro-rodríguez V (2023). Monitoring nutrients in plants with genetically encoded sensors: Achievements and perspectives.
Avila C et al. Identification of metabolic pathways differentially regulated in somatic and zygotic embryos of maritime pine .doi: 10.3389/978-2-8325-25.
El-Azaz J, Cánovas FM, Barcelona B, Ávila C, de la Torre F. Deregulation of phenylalanine biosynthesis evolved with the emergence of vascular plants.
Valderrama-Martín JM, Ortigosa F, Ávila C, Cánovas FM, Hirel B, Cantón FR, Cañas RA. A revised view on the evolution of glutamine synthetase isoenzymes in plants. doi: 10.1111/tpj.15712.
Ávila C, Llebrés MT, Castro-Rodríguez V, Lobato-Fernández C, Reymond I, Harvengt L, Trontin JF, Cánovas FM. Identification of Metabolic Pathways Differentially Regulated in Somatic and Zygotic Embryos of Maritime Pine. doi: 10.3389/fpls.2022.877960.
Ortigosa, F.; Ávila, C.; Rubio, L.; Álvarez-Garrido, L.; Carreira, J.A.; Cañas, R.A.; Cánovas, F.M. Transcriptome Analysis and Intraspecific Variation in Spanish Fir (Abies pinsapo Boiss.) .Int. J. Mol. Sci. 2022 , 23 , 9351. https://doi.org/10.3390/ijms23169351
Llebrés MT, Castro-Rodríguez V, Pascual MB, Avila C, Cánovas FM. The amino acid permease PpAAP1 mediates arginine transport in maritime pine. Tree Physiol. doi: 10.1093/treephys/tpab089.
Ortigosa F, Lobato-Fernández C, Pérez-Claros JA, Cantón FR, Ávila C, Cánovas FM, Cañas RA. Epitranscriptome changes triggered by ammonium nutrition regulate the proteome response of maritime pine roots. doi: 10.3389/fpls.2022.1102044.
Ortigosa F, Lobato-Fernández C, Shikano H, Ávila C, Taira S, Cánovas FM, Cañas RA. Ammonium regulates the development of pine roots through hormonal crosstalk and differential expression of transcription factors in the apex. doi: 10.1111/pce.14214.
Ávila C, Cañas RA, de la Torre F, Pascual MB, Castro-Rodríguez V, Cantón FR, Cánovas FM (2022). Functional Genomics of Mediterranean pines. https://doi.org/10.1007
Sterck L, de María N, Cañas RA, de Miguel M, Perdiguero P, Raffin A, Budde KB, López-Hinojosa M, Cantón FR, Rodrígues AS, Morcillo M, Hurel A, Vélez MD, de la Torre F, Modesto I, Manjarrez LF, Pascual MB, Alves A, Mendoza-Poudereux I, Cllejas Diaz M, Pizarro A, El-Azaz J, Hernández-Escribano L, Guevara MA, Majada J, Salse J, Grivet D, Bouffier L, Raposo R, de la Torre AR, Zas R, Cabezas JA, Ávila C, Trontin J-F, Sánchez L, Alía R, Arrillaga I, González-Martín SC, Miguel C, Cánovas FM, Plomion C, Díaz-Sala C, Cervera MT (2022). Maritime Pine Genomics in Focus. https://doi.org/10.1007/978-3-030-93390-6_5
Castro-Rodríguez V, Kleist TJ, Gappel, NM, Atanjaoui F, Okumoto S, Machado M,Denyer T, Timmermans MCP, Frommer, WB, and Wudick, MM. (2022) Sponging of glutamate at the outer plasma membrane surface reveals roles for glutamate in development.
Llebrés MT, Pascual MB, Valle C, de la Torre FN, Valderrama-Martin JM, Gómez L, Avila C and Cánovas FM. Structural and Functional Characteristics of Two Molecular Variants of the Nitrogen Sensor PII in Maritime Pine. Frontiers in Plant Science 2020, 11:823. doi: 10.3389/fpls.2020.00823
El-Azaz J, de la Torre F, Pascual MB, Debille S, Canlet F, Harvengt L, Trontin JF, Ávila C, Cánovas FM.Transcriptional Analysis of Arogenate Dehydratase Genes Identifies a Link Between Phenylalanine Biosynthesis and Lignin Biosynthesis. J Exp Bot. 2020, 71:3080-3093. doi: 10.1093/jxb/eraa099
Ortigosa F, Valderrama-Martín JM, Urbano-Gámez JA, García-Martín ML, Ávila C, Cánovas FM, Cañas RA. Inorganic Nitrogen Form Determines Nutrient Allocation and Metabolic Responses in Maritime Pine Seedlings. Plants. 2020, 9:481. doi: 10.3390/plants9040481
Cañas RA, Yesbergenova-Cuny Z, Belanger L, Rouster J, Brulé L, Gilard F, Quilleré I, Sallaud C, Hirel B. NADH-GOGAT Overexpression Does Not Improve Maize (Zea mays L.) Performance Even When Pyramiding with NAD-IDH, GDH and GS. Plants. 2020, 9:130. doi: 10.3390/plants9020130
Urbano-Gámez JA, El-Azaz J, Ávila C, de la Torre FN, Cánovas FM. Enzymes Involved in the Biosynthesis of Arginine from Ornithine in Maritime Pine (Pinus pinaster Ait.). doi: 10.3390/plants9101271.
Castro-Rodríguez V, Ávila C, Cánovas FM. Getting more bark for your buck: nitrogen economy of deciduous forest trees doi: 10.1093/jxb/eraa238.
Sadoine M, Castro-Rodríguez V, Poloczeck T, Javot H, Sunal E and Frommer WB. Purification of genetically encoded His-tagged GO Matryoshka biosensors for analysis of ligand-protein interactions by using in vitro binding assays (2020 )
Ortigosa F, Valderrama-Martín JM, Ávila C, Cánovas FM, Cañas RA. Understanding Plant Nitrogen Nutrition through a Laboratory Experiment. Biochemistry and Molecular Biology Education. 2019, 47(4):450-458 doi: 10.1002/bmb.21239
Cañas RA, Pascual MB, de la Torre FN, Ávila C, Cánovas FM. Resources for conifer functional genomics at the omics era .In Molecular Physiology and Biotechnology of Trees. Advances in Botanical Research. 2019, vol. 89:39-76. Academic Press, Londres. doi: 10.1016/bs.abr.2018.11.002
Pascual MB, de la Torre F, Cañas RA, Cánovas FM, Ávila C. NAC Transcription Factors in Woody Plants. Progress in Botany 2019, Springer, Berlin, Heidelberg. vol 80:195-222. doi: 10.1007/124_2018_19
El-Azaz J, Cánovas FM, Ávila C, de la Torre F. The arogenate dehydratase ADT2 is essential for seed development in Arabidopsis. Plant and Cell Physiology 2018, 59:2409-2420. doi: 10.1093/pcp/pcy200
Cánovas FM, Cañas RA, de la Torre FN, Pascual MB, Castro-Rodríguez V, Avila C. Nitrogen Metabolism and Biomass Production in Forest Trees. Frontiers in Plant Science 2018, 9:1449. doi: 10.3389/fpls.2018.01449.
Llebrés MT, Avila C, Cánovas FM, Klimaszewska K. Root growth of somatic plants of hybrid Pinus strobus (L.) and P. wallichiana (A. B. Jacks.) is affected by the nitrogen composition of the somatic embryo germination medium. Trees 2018, 32(2): 371-381. doi: 10.1007/s00468-017-1635-2
Llebrés MT, Pascual MB, Debille S, Trontin JF, Harvengt L, Avila C, Cánovas FM. The role of arginine metabolic pathway during embryogenesis and germination in maritime pine (Pinus pinaster Ait.) Tree Physiology 2018, 38(3):471-484. doi: 10.1093/treephys/tpx133
Pascual MB, Llebrés MT, Craven-Bartle B, Cañas RA, Cánovas FM, Avila C.PpNAC1, a main regulator of phenylalanine biosynthesis and utilization in maritime pine. Plant Biotechnology Journal 2018, 16(5):1094-1104. doi: 10.1111/pbi.12854
García-Gutiérez A, Cánovas FM, Avila C. Glutamate synthases from conifers: gene structure and phylogenetic studies. BMC Genomics 2018, 19(1):65. doi:10.1186/s12864-018-4454-y
Alvarez JM, Bueno N, Cañas RA, Avila C, Cánovas FM, Ordás RJ. Analysis of the WUSCHEL-RELATED HOMEOBOX gene family in Pinus pinaster : New insights into the gene family evolution. Plant Physiology and Biochemistry 2018, 123:304-318. doi:10.1016/j.plaphy.2017.12.031
Cañas RA, Li Z, Pascual MB, Castro-Rodríguez V, Avila C, Sterck L, Van de Peer Y, Cánovas FM. The gene expression landscape of pine seedling tissues .
Van Kerckhoven SH, de la Torre FN, Cañas RA, Avila C, Cantón FR, Cánovas FM. Characterization of three L-asparaginases from maritime pine (Pinus pinaster Ait) .
Jauregui I, Aparicio-Tejo PM, Baroja E, Avila C, Aranjuelo I. Elevated CO2 improved the growth of a double nitrate reductase defective mutant of Arabidopsis thaliana: the importance of maintaining a high energy status . Environ Exp Bot. 2017, 140: 110-119. doi: 10.1016/j.envexpbot.2017.06.003
Li Z, De La Torre AR, Sterck L, Canovas FM, Avila C, Merino Sierra I, Antonio Cabezas J, Teresa Cervera M, Ingvarsson PK, Van de Peer Y.Single-copy genes as molecular markers for phylogenomic studies in seed plants.
Rueda-López M, Pascual MB, Pallero M, Henao LM, Lasa B, Jauregui I, Aparicio-Tejo PM, Cánovas FM, Ávila C. Overexpression of a pine Dof transcription factor in hybrid poplars: A comparative study in trees growing under controlled and natural conditions.
Castro-Rodríguez V, Cañas RA, de la Torre FN, Pascual MB, Avila C, Cánovas FM. Molecular fundamentals of nitrogen uptake and transport in trees.
Merino I, Abrahamsson M, Sterck L, Craven-Bartle B, Cánovas FM, von Arnold S. Transcript profiling for early stages during embryo development in Scots pine.
Pascual B, El-Azaz J, de la Torre FN, Cañas RA, Avila C, Cánovas FM.Biosynthesis and metabolic fate of phenylalanine in conifers.
Granados JM, Ávila C, Cánovas FM, Cañas RA. Selection and testing of reference genes for accurate RT-qPCR in adult needles and seedlings of maritime pine.
El-Azaz J, de la Torre F, Ávila C, Cánovas FM. Identification of a small protein domain present in all plant lineages that confers high prephenate dehydratase activity.
Zarei A, Zamani Z, Fatahi R, Mousavi A, Salami SA, Avila C, Cánovas FM. Differential expression of cell wall related genes in the seeds of soft- and hard seeded pomegranate genotypes.
Cañas RA, de la Torre F, Pascual MB, Avila C, Cánovas FM. Nitrogen Economy and Nitrogen Environmental Interactions in Conifers.
Seoane-Zonjic P, Cañas RA, Bautista R, Gómez-Maldonado J, Arrillaga I, Fernández-Pozo N, Claros MG, Cánovas FM, Ávila C. Establishing gene models from the Pinus pinaster genome using gene capture and BAC sequencing.
Jauregui I, Aparicio-Tejo PM, Avila C, Cañas R, Sakalauskiené S, Aranjuelo I. Root-shoot interactions explain the reduction of leaf mineral content in Arabidopsis plants grown under elevated [CO(2)] conditions.
Castro-Rodriguez V, Garcia-Gutierrez A, Canales J, Cañas RA, Kirby E, Avila C, Canovas FM. Poplar trees for phytoremediation of high levels of nitrate and applications in bioenergy.
Castro-Rodríguez V, Assaf-Casals I, Pérez-Tienda J, Fan X, Avila C, Miller A, Cánovas FM. Deciphering the molecular basis of ammonium uptake and transport in maritime pine .
Cañas RA, Feito I, Fuente-Maqueda JF, Ávila C, Majada J, Cánovas FM.Transcriptome-wide analysis supports environmental adaptations of two Pinus pinaster populations from contrasting habitats.
Pascual MB, Cánovas FM, Ávila C. The NAC transcription factor family in maritime pine (Pinus Pinaster): molecular regulation of two genes involved in stress responses.
Rueda-López M, Cañas RA, Canales J, Cánovas FM, Ávila C. The over-expression of the pine transcription factor PpDof 5 in Arabidopsis leads to increased lignin content and affects carbon and nitrogen metabolism.
Cañas RA, Canales J, Muñoz C, Granados JM, Avila C, García-Martín ML, Cánovas FM. Understanding developmental and adaptive cues in pine through metabolite profiling and co-expression network analysis.
Castro-Rodríguez V, García-Gutiérrez A, Cañas RA, Pascual M, Avila C, Cánovas FM. Redundancy and metabolic function of the glutamine synthetase gene family in poplar.
Fernando de la Torre, Rafael A. Cañas, M. Belén Pascual, Concepción Avila, Francisco M. Cánovas. Plastidic aspartate aminotransferases and the biosynthesis of essential amino acids in plants.
Cañas RA, Canales J, Gómez-Maldonado J, Ávila C, Cánovas FM. Transcriptome analysis in maritime pine using laser capture microdissection and 454 pyrosequencing.
Canales J, Bautista R, Label P, Gómez-Maldonado J, Lesur I, Fernández-Pozo N, Rueda-López M, Guerrero-Fernández D, Castro-Rodríguez V, Benzekri H, Cañas RA, Guevara MA, Rodrigues A, Seoane P, Teyssier C, Morel A, Ehrenmann F, Le Provost G, Lalanne C, Noirot C, Klopp C, Reymond I, García-Gutiérrez A, Trontin JF, Lelu-Walter MA, Miguel C, Cervera MT, Cantón FR, Plomion C, Harvengt L, Avila C, Gonzalo Claros M, Cánovas FM. De novo assembly of maritime pine transcriptome: implications for forest breeding and biotechnology.
Merino I, Contreras A, Jing ZP, Gallardo F, Cánovas FM, Gómez L. Plantation forestry under global warming: hybrid poplars with improved thermotolerance provide new insights on the in vivo function of small heat shock protein chaperones .doi: 10.1104/pp.113.225730. 164(2):978-91
de la Torre FN, El-Azaz J, Avila C, Canovas FM. Deciphering the role of aspartate and prephenate aminotransferase activities in plastid nitrogen metabolism.
Flores-Monterroso A, Canales J, de la Torre F, Ávila C, Cánovas FM.Identification of genes differentially expressed in ectomycorrhizal roots during the Pinus pinaster-Laccaria bicolor interaction.
Craven-Bartle B, Pascual MB, Cánovas FM, Avila C. A Myb transcription factor regulates genes of the phenylalanine pathway in maritime pine. Plant J 2013, doi: 10.1111/tpj.12158. 74(5):755-66
Rueda-López M, García-Gutiérrez A, Cánovas FM, Ávila C. The family of Dof transcription factors in pine.
Mackay J., Dean J.F., Plomion C., Peterson D.G., Cánovas F.M. Pavy N., Ingvarsson P.K., Savolainen O., Guevara M.A., Fluch S., Vinceti B., Abarca D., Díaz-Sala C., Cervera M.T. Towards decoding the conifer giga-genome.
Canales J., Rueda-López M., Craven-Bartle B., Avila C., Cánovas F.M.Novel insights into regulation of asparagine synthetase in conifers
Coleman H.D., Cánovas F.M., Man H., Kirby E.G., Mansfield S.D.Enhanced expression of glutamine synthetase (GS1a) confers altered fibre and wood chemistry in field grown hybrid poplar (Populus tremula X alba) (717-1B4).
Villalobos D.P., Díaz-Moreno S.M., Said el-SS, Cañas R.A., Osuna D., Van Kerckhoven S.H., Bautista R., Claros M.G., Cánovas F.M., Cantón F.R.Reprogramming of gene expression during compression wood formation in pine: coordinated modulation of S-adenosylmethionine, lignin and lignan related genes.
Canales J., Avila C., Cantón F.R., Villalobos D.P., Díaz-Moreno S., Ariza D., Molina-Rueda J.J., Navarro-Cerrillo R.M., Claros M.G., Cánovas F.M.Gene expression profiling in the stem of young maritime pine trees: detection of ammonium stress-responsive genes in the apex.
Castro-Rodríguez V., García-Gutiérrez A., Canales J., Avila C., Kirby E., Cánovas F.M. The glutamine synthetase gene family in Populus .
Fernández-Pozo N., Canales J., Guerrero-Fernández D., Villalobos D.P., Díaz-Moreno S., Bautista R., Flores-Monterroso A., Guevara M.A., Perdiguero P., Collada C., Cervera M.T., Soto A., Ordás R., Cantón F.R., Avila C., Cánovas F.M. and Claros M.G. EuroPineDB: a high-coverage Web database for maritime pine transcriptome .
Canales J., Avila C., Cánovas F.M. A maritime pine antimicrobial peptide involved in ammonium nutrition .
Sanz-Santos G., Jiménez-Marín A., Bautista R., Fernández-Pozo N., Claros M.G., Garrido J.J. (2011) Gene expression pattern in swine neutrophils after lipopolysaccharide exposure: a time course comparison.
Falgueras J., Lara A.J., Pérez-Trabado G., Fernández-Pozo N., Cantón F.R. and Claros M.G. (2010) SeqTrim07: a pipeline for preprocessing sequence reads.
Molina-Rueda J.J., Pascual M.B., Cánovas F.M. and Gallardo F. (2010)Characterization and developmental expression of a glutamate decarboxylase from maritime pine .
Guerrero D., Bautista R., Villalobos D.P., Cantón F.R., Claros M.G. (2010)AlignMiner: a Web-based tool for detection of divergent regions in multiple sequence alignments of conserved sequences.
Ramírez S., Muñoz-Merida A., Karlsson J., García M., Pérez-Pulido A.J., Claros M.G. and Trelles O. (2010) MOWServ: a web client for integration of bioinformatic resources.
Canales J., Flores-Monterroso A., Rueda-López M., Avila C., Cánovas F.M. (2010) Identification of genes regulated by ammonium availability in the roots of maritime pine trees.
Falgueras J., Lara A.J., Fernández-Pozo N., Cantón F.R., Pérez-Trabado G., Claros M.G.(2010). SeqTrim: a high-throughput pipeline for preprocessing any type of sequence reads.
Claros M.G., Aguilar M., Cánovas F.M. (2010) Evidence for an operative glutamine translocator in chloroplasts from maritime pine (Pinus pinaster Ait.) cotyledons.
Jens F. Sundström, Alena Vaculova, Andrei P. Smertenko, Eugene I. Savenkov, Anna Golovko, Elena Minina, Budhi S. Tiwari, Salvador Rodriguez-Nieto, Andrey A. Zamyatnin, Tuuli Välineva, Juha Saarikettu, Mikko J. Frilander, Maria F. Suarez, Anton Zavialov, Ulf Ståhl, Patrick J. Hussey, Olli Silvennoinen, Eva Sundberg, Boris Zhivotovsky & Peter V. Bozhkov (2009). Tudor staphylococcal nuclease is an evolutionarily conserved component of the programmed cell death degradome.
Cánovas F.M., Canales F.J., Flores A., Rueda-López M., Avila C. (2009)Functional genomics approaches to study ammonium nutrition and amino acid biosynthesis in conifers.
Rodriguez-Llorente I., Caviedes M.A., Dary M., Palomares A., Cánovas F.M., Peregrin-Alvarez J.M. (2009) The Symbiosis Interactome: a computational system biology approach reveals novel components, functional interactions and modules in Sinorhizobium meliloti.
De la Torre, F. and Moya-Garcia, A. A. and Suarez, M. F. and Rodriguez-Caso, C. and Canas, R. A. and Sanchez-Jimenez, F. and Canovas, F. M. (2009). Molecular modeling and site-directed mutagenesis reveal essential residues for catalysis in a prokaryote-type aspartate aminotransferase.
El Omari R., Rueda-López M., Avila C., Nhiri M., Cánovas F.M. (2009)Ammonium tolerance and the regulation of two cytosolic glutamine synthetases in the roots of Sorghum .
Martin-Requena, V. and Munoz-Merida, A. and Claros, M. G. and Trelles, O. (2009). PreP+07: improvements of a user friendly tool to preprocess and analyse microarray data.
J.P. Florido, H. Pomares, I. Rojas, J.C. Calvo, J.M. Urquiza, and Claros M.G. (2009). On Selecting the Best Pre-processing Method for Affymetrix Genechips.
David Díaz, Sergio Gálvez, Juan Falgueras, Juan Antonio Caballero, Pilar Hernández, Gonzalo Claros, and Gabriel Dorado (2009). Intuitive Bioinformatics for Genomics Applications: Omega-Brigid Workflow Framework.
Rueda-López M, Crespillo R, Cánovas FM, Avila C. (2008)Differential regulation of two glutamine synthetase genes by a single Dof transcription factor .
Cañas, R. A. and Villalobos, D. P. and Diaz-Moreno, S. M. and Canovas, F. M. and Canton, F. R. (2008) Molecular and functional analyses support a role of Ornithine-[delta]-aminotransferase in the provision of glutamate for glutamine biosynthesis during pine germination.
María Belén Pascual, Zhong Ping Jing, Edward G. Kirby, Francisco M. Cánovas, Fernando Gallardo. (2008) Response of transgenic poplar overexpressing cytosolic glutamine synthetase to phosphinothricin.
M. Belén Pascual, Juan Jesús Molina-Rueda, Francisco M. Cánovas and Fernando Gallardo (2008) Spatial distribution of cytosolic NADP+ isocitrate dehydrogenase in pine embryos and seedlings.
Rocío Bautista, David P. Villalobos, Sara Díaz-Moreno, Francisco R. Cantón, Francisco M. Cánovas, M. Gonzalo Claros (2008)New strategy for pinus pinaster genomic library construction in bacterial artificial chromosomes.
Wilkinson, M. D., (…) Gelpi, J. L., Trelles, O., (…) Carazo, J. M., Dopazo, J., Guigo, R., (…) Orozco, M., Valencia, A., Claros, M. G., (…) Aldana, J., (…) and Groscurth, A. (2008) Interoperability with Moby 1.0–it’s better than sharing your toothbrush! Brief Bioinformatics 9, 220–231
Cánovas FM, Avila C, Cantón FR, Gallardo F (2008) Molecular biology and biotechnology of tree nitrogen metabolism. Recent Advances in Biotechnology. Eds. BN Prasad, L Mathew. Excel India Publishers, New Delhi, India, pp 58-62.
Rueda M, Crespillo R, Cánovas FM, Avila C (2008) Regulation of ammonium assimilation in pine trees. Gutamine synthetase genes putative targets of Dof trasncription factors. Recent Advances in Biotechnology. Eds. BN Prasad, L Mathew. Excel India Publishers, New Delhi, India, pp 77-81.
De la Torre, F. and Suarez, M. F. and Santis, L. and Canovas, F. M. (2007)The aspartate aminotransferase family in conifers: biochemical analysis of a prokaryotic-type enzyme from maritime pine.
Alonso, P. and Cortizo, M. and Canton, F. R. and Fernandez, B. and Rodriguez, A. and Centeno, M. L. and Canovas, F. M. and Ordas, R. J. (2007) Identification of genes differentially expressed during adventitious shoot induction in Pinus pinea cotyledons by subtractive hybridization and quantitative PCR.
Goncalves, S. and Cairney, J. and Rodriguez, M. P. and Canovas, F. and Oliveira, M. and Miguel, C. (2007) PpRab1, a Rab GTPase from maritime pine is differentially expressed during embryogenesis.
Canas, R. A. and de la Torre, F. and Canovas, F. M. and Canton, F. R. (2007) Coordination of PsAS1 and PsASPG expression controls timing of re-allocated N utilization in hypocotyls of pine seedlings .
Canovas, F. M. and Avila, C. and Canton, F. R. and Canas, R. A. and de la Torre, F. (2007) Ammonium assimilation and amino acid metabolism in conifers.
Rocío Bautista, David P. Villalobos, Sara Díaz-Moreno, Francisco R. Cantón, Francisco M. Cánovas, M. Gonzalo Claros (2007) Toward a Pinus pinaster bacterial artificial chromosome library.
Baumann, K. and Perez-Rodriguez, M. and Bradley, D. and Venail, J. and Bailey, P. and Jin, H. and Koes, R. and Roberts, K. and Martin, C. (2007)Control of cell and petal morphogenesis by R2R3 MYB transcription factors.
Lara A.J., Pérez-Trabado G., Villalobos D.P., Díaz-Moreno S., Cantón F.R., Claros M.G. (2007) A Web Tool to Discover Full-Length Sequences: Full-Lengther.
Falgueras J., Lara A.J., Cantón F.R., Pérez-Trabado G., Claros M.G. (2007) SeqTrim: a Validation and Trimming Tool for All Purpose Sequence Reads. Amine System Project.
F. de la Torre, L. De Santis, M.F. Suárez, R. Crespillo, F.M. Cánovas (2006) Identification and functional analysis of a prokaryotic-type aspartate aminotransferase: implications for plant amino acid metabolism. Plant Journal. 46, 414–425
Ávila C., Pérez-Rodríguez J., Cánovas F.M. (2006) Molecular characterization of a receptor-like protein kinase gene from pine (Pinus sylvestris L.).
R.A. Cañas, F. de la Torre, F.M. Cánovas, F.R. Cantón (2006) High levels of asparagine synthetase in hypocotyls of pine seedlings suggest a role of the enzyme in re-allocation of seed-stored nitrogen. Planta 224, 83–95
Reyes, D. and Rodriguez, D. and Lorenzo, O. and Nicolas, G. and Canas, R. and Canton, F. R. and Canovas, F. M. and Nicolas, C. (2006) Immunolocalization of FsPK1 correlates this abscisic acid-induced protein kinase with germination arrest in Fagus sylvatica L. seeds.
Pérez-Rodíguez M. J, Suárez M. F., Heredia R., Ávila C., Breton D., Trontin J. F., Filonova L., Bozhkov P., von Arnold S., Harvengt L., Cánovas F. M. (2006) Expression patterns of two glutamine synthetase genes in zygotic and somatic pine embryos support specific roles in nitrogen metabolism during embryogenesis.
D.Reyes, D. Rodríguez, O. Lorenzo, G. Nicolás, R. Cañas, F.R. Cantón, F.M. Cánovas, C. Nicolás (2006) Immunolocalization of FsPK1 correlates this abscisic acid-induced protein kinase with germination arrest in Fagus sylvatica L. seeds.
S.Tereso, C.Miguel, K.Zoglauer, C.Valle, M.Margarida (2006) Stable agrobacterium-mediated transformation of embryogenic tissues from pinus pinaster portuguese genotypes .
Kirby E.G., Gallardo F., Man H., El-Khatib R. (2006) The overexpression of glutamine synthetase in transgenic poplar: a review. Silvae Genetica 55, 278–284
Cánovas FM, Gallardo F Jing ZP, Pascual B (2006) Transgenic approaches to engineer nitrogen metabolism. Tree Transgenesis. Recent Developments. Eds M Fladung, S Ewald, Springer Verlag, Heildelberg, Germany pp 157-178.
Gallardo F, Gómez L, Cánovas FM (2006) Arboles transgénicos Organismos modificados genéticamente. Ed E Muñoz, Editorial Ephemera, capítulo 15, pags. 281-293
Butland, G. and Peregrin-Alvarez, J. M. and Li, J. and Yang, W. and Yang, X. and Canadien, V. and Starostine, A. and Richards, D. and Beattie, B. and Krogan, N. and Davey, M. and Parkinson, J. and Greenblatt, J. and Emili, A. (2005). Interaction network containing conserved and essential protein complexes in Escherichia coli.
Peregrin-Alvarez, J. M. and Yam, A. and Sivakumar, G. and Parkinson, J. (2005) PartiGeneDB––collating partial genomes.
Canton FR, Suarez MF, Canovas FM. (2005) Molecular aspects of nitrogen mobilization and recycling in trees. Photosynthesis Research. 83, 265-278
Bozhkov P, Filonova L, Suarez MF. Programmed cell death in plant embryogenesis (2005) Current Topics in Developmental Biology 67, 135-179.
Bozhkov P, Suarez MF, Filonova L. Cysteine protease MCII-PA executes programmed cell death during plant embryogenesis (2005) Proceedings of the National Academy of Sciences (PNAS) USA, 102, 14463-14468.
Teixeira, J. and Pereira, S. and Canovas, F. and Salema, R. (2005)Glutamine synthetase of potato (Solanum tuberosum L. cv. Desiree) plants: cell- and organ-specific expression and differential developmental regulation reveal specific roles in nitrogen assimilation and mobilization
Perez-Rodriguez, M. and Jaffe, F. W. and Butelli, E. and Glover, B. J. and Martin, C. (2005) Development of three different cell types is associated with the activity of a specific MYB transcription factor in the ventral petal of Antirrhinum majus flowers. Development 132: num.2 359-370.
Pereira-Leal, J. B. and Audit, B. and Peregrin-Alvarez, J. M. and Ouzounis, C. A. (2005). An exponential core in the heart of the yeast protein interaction network.
Jing ZP, Gallardo Pascual B, Cánovas FM (2005). Improved growth of transgenic hybid poplar overexpressing glutamine synthetase. Plant Nutrition for food security, human health and environmental protection. Eds Li CJ, Zhang FS, Doberman A, et al., Tsinghua University press, Beijing, China, pp 142-143.
Gomez-Maldonado J, Avila C, Torre F, Canas R, Canovas FM, Campbell MM (2004). Functional interactions between a glutamine synthetase promoter and MYB proteins.
Villalobos D. P., R. Bautista, F. M. Cánovas, M. G. Claros (2004)Isolation of Bacterial Artificial Chromosome DNA by Means of Improved Alkaline Lysis and Double Potassium Acetate Precipitation.
Ping Jing Z, Gallardo F, Pascual MB, Sampalo R, Romero J, Torres de Navarra A, Cánovas FM (2004). Improved growth in a field trial of transgenic hybrid poplar overexpressing glutamine synthetase. New Phytologist 164, 137–145
Gómez-Maldonado J, Cánovas FM, Avila C (2004). Molecular analysis of the 5′ upstream region of a gibberellin-inducible cytosolic glutamine synthetase gene (GS1b) expressed in the pine vascular tissue.
El-Khatib, R, Hamerlynck, EP, Gallardo F, Kirby EG. (2004)Transgenic poplar characterized by ectopic expression of a pine cytosolic glutamine synthetase gene exhibits enhanced tolerance to water stress.
Gómez-Maldonado J, Avila C, Barnestein P, Crespillo R, Cánovas FM. (2004). Interaction of cis-acting elements in the expression of a cytosolic glutamine synthetase gene in pine seedlings.
De la Torre F, R. Bautista, F.M. Canovas, M.G. Claros (2004)Isolation of DNA from olive oil: applications in DNA fingerprinting with SCAR markers.
Sahrawy M, Avila C, Chueca A, Canovas FM, Lopez-Gorge J. (2004)Increased sucrose level and altered nitrogen metabolism in Arabidopsis thaliana transgenic plants expressing antisense chloroplastic fructose-1,6-bisphosphatase.
Sahrawy M, Chueca A, Canovas FM, Lopez-Gorge J. (2004)Role of chloroplastic fructose-1,6-bisphosphatase in sucrose synthesis
Francisco M. Cánovas, Eliane Dumas-Gaudot, Ghislaine Recorbet, Jesus Jorrin, Hans-Peter Mock, Michel Rossignol (2004)Plant proteome analysis .
Stasolla C, Bozkov PV, Chu T-M, von Zyl L, Egertsdotter U, Suárez MF, Craig D, Wolfinger RD, von Arnold S, Sederoff RR. (2004)Variation in transcript abundance during somatic embryogenesis in gymnosperms .
Bozkov PV, Filonova LH, Suárez MF, Helmerson A, Zhivotovsky B, Smertenko A, von Arnold S (2004). VEIDase is a principal caspase-like activity implicated in developmental programmed cell death during plant embryogenesis.
Olea F, Pérez-García A, Avila C, Cantón FR, Cazorla F, Rivera E, Cánovas FM, de Vicente A. (2004) Up-regulation and localization of asparagine synthetase in tomato leaves infected by the bacterial pathogen Pseudomonas syringae.
Suárez MF, Filonova LH, Smertenko AP, Savenkov EI, Clapham DH, von Arnold S, Zhivotosky B, Bozhkov PV (2004). Metacaspase-dependent programmed cell death is essential for embryogenesis.
González-Halphen D, S. Funes, X. Pérez-Martínez, A. Reyes-Prieto, M.G. Claros, E. Davidson, M.P. King (2004). Genetic correction of mitochondrial diseases: using the natural migration of mitochondrial genes to the nucleus in chlorophyte algae as a model system.
Fu J, Sampalo R, Gallardo F, Cánovas FM, Kirby EG (2003). Assembly of a cytosolic pine glutamine synthetase holoenzyme in leaves of transgenic poplar leads to enhanced vegetative growth in young plants.
Gallardo F, Fu J, Jing ZP, Kirby EG, Cánovas FM (2003). Genetic modification of amino acid metabolism in woody plants.
R. Bautista, F.M. Canovas, M.G. Claros (2003). Genomic evidence for a repetitive nature of the RAPD polymorphisms in Olea europaea (olive-tree)
Santillan Torres, J. L. and Atteia, A. and Claros, M. G. and Gonzalez-Halphen, D. (2003). Cytochrome f and subunit IV, two essential components of the photosynthetic bf complex typically encoded in the chloroplast genome, are nucleus-encoded in Euglena gracilis. Biochim Biophys Acta 1604, 180–189
Janssen, P. and Audit, B. and Cases, I. and Darzentas, N. and Goldovsky, L. and Kunin, V. and Lopez-Bigas, N. and Peregrin-Alvarez, J. M. and Pereira-Leal, J. B. and Tsoka, S. and Ouzounis, C. A. (2003). Beyond 100 genomes.
Peregrin-Alvarez, J. M. and Tsoka, S. and Ouzounis, C. A. (2003). The phylogenetic extent of metabolic enzymes and pathways.
R. Bautista, R. Crespillo, F.M. Canovas, M.G. Claros (2002). Identification of olive-tree cultivars with SCAR markers.
Martínez, Jaime F.; Monte, Elena y Ruiz Cantón, F. J. Fitocromos y desarrollo vegetal. Scientific American 305 –February 2002
Suárez M.F. , C. Ávila, F. Gallardo, F.R. Cantón, A. García-Gutiérrez, M.G. Claros, F.M. Cánovas (2002). Molecular and enzymatic analysis of ammonium assimilation in woody plants. Journal of Experimental Botany . 53, 891-904
Avila C, Cantón FR, Barnestein P, Suárez M-F, Marraccini P, Rey M, Humara JM Ordás R y Cánovas FM. (2002). Structural an functional characterization of the 5´upstream region of a glutamine synthetase gene from Scots pine. Ann. For. Sci. 59, 669-673
de la Torre F, García-Gutiérrez A, Crespillo R, Cantón FR, Avila C, Cánovas FM (2002). Functional expression of two pine glutamine synthetase genes in bacteria reveals that they encode cytosolic holoenzymes with different molecular and catalytic properties. Plant Cell Physiol 43, 802-809.
Avila C, Cantón FR, Barnestein P, Suárez MF, Marraccini P, Rey M, Humara JM, Ordás R, Cánovas FM (2001). The promoter of a cytosolic glutamine synthetase gene from the conifer Pinus sylvestris is active in cotyledons of germinating seeds and light-regulated in transgenic Arabidopsis thaliana. Physiologia Plantarum 112, 338-396.
Avila C, M Suárez MF, Gómez-Maldonado J, Cánovas FM (2001). Spatial and temporal expression of two cytosolic glutamine synthetase genes in Scots pine: functional implications on nitrogen metabolism during early stages of conifer development.
Gomez-Maldonado J, Crespillo R, Avila C, Cánovas FM (2001). Efficient preparation of protoplasts from maritime pine (pinus pinaster) suitable for transgene expression analysis.
Cánovas FM, Florencio FJ (2000). Avances en el Metabolismo del Nitrógeno: Bioquímica, Fisiología y Biología Molecular . Servicio de Publicaciones, Universidad de Málaga.
Claros, M.G., R. Crespillo, M.L. Aguilar & F.M. Cánovas, 2000.DNA fingerprinting and classification of geographically related genotypes of olive-tree (Olea europaea L.) .
Avila C, Muñoz-Chápuli R, Plomion C., Frigerio J.M., Cánovas F.M. (2000). Two Genes Encoding Distinct Cytosolic Glutamine Synthetases Are Closely Linked in the Pine Genome.
Avila C, Cánovas FM (2000). An Improved and Rapid Protocol for the Isolation of poly(A)+ -RNA from Small Samples of Scots Pine Seedlings .
Cantón, FR, Suárez, MF, Josè-Estayol, M, Cánovas, FM (1999). Expression analysis of a cytosolic glutamine synthetase gene in cotyledons of Scots pine seedlings: developmental, light/dark regulation and spatial distribution of specific transcripts.
Gallardo F, Fu J, Cantón FR, García-Gutiérrez A, Cánovas FM, Kirby EG (1999). Expression of a conifer glutamine synthetase gene in transgenic poplar.
Plomion, C, Hurme, P, Frigerio, JM, Ridolfi, M, Pot, D, Pionneau, C, Avila, C, Gallardo, F, David, H, Neutlings, G, Campbell, M, Cánovas, FM, Savolainen, O, Bodénès C, Kremer, A (1999). Developing SSCP markers in two Pinus species.
García-Gutiérrez, A., Dubois, F., Cantón, F.R., Gallardo, F., Sangwan, S., Cánovas, F.M. (1998). Two different models of early development and nitrogen assimilation in gymnosperm seedlings .
Claros, MG, Cánovas, FM (1998). Rapid high quality RNA preparation from pine seedlings . Plant Mol. Biol. Rept. 16, 9-18.
Cánovas FM, Cantón FR, García-Gutiérrez A, Gallardo F, Crespillo R (1998). Molecular physiology of glutamine and glutamate biosynthesis in developing seedlings of conifers. Physiol. Plantarum, 103, 287-294
Palomo, J, Gallardo, F, Suárez, MF, Cánovas, FM (1998). Purification and characterization of NADP+ -linked isocitrate dehydrogenase from Scots pine.
Avila, C, García-Gutiérrez, A, Crespillo, R, Cánovas, FM (1998)Effects of phosphinotricin treatment on glutamine synthetase isoforms in Scots pine seedlings.
Pérez-García A, de Vicente A, Cantón F.R, Cazorla FM, Codina JC, García-Gutiérrez A, Cánovas FM (1998). Light-dependent changes of tomato glutamine synthetase in response to Pseudomonas syringae infection or phosphinothricin treatment. 10.1034/j.1399-3054.1998.1020306.x
Claros MG, Cánovas FM (1998). Lineal kinetics: a graphic tool to deal wih linear regressions. Embnet News 5, 7-9
Pérez-García A, Pereira S, Pisarra J, García-Gutiérrez A, Cazorla F, Salema R, de Vicente A, Cánovas FM (1998). Cytosolic localization in tomato mesophyll cells of a novel glutamine synthetase induced in response to bacterial infection or phosphinothricin treatment. 10.1007/s004250050418
García-Gutiérrez A, Cantón FR, Sánchez-Jiménez F, Gallardo F, Cánovas FM (1995). Expression of ferredoxin-dependent glutamate synthase in dark-grown pine seedlings .10.1007/BF00019183
Gallardo F, Gálvez S, Gadal P, Cánovas FM (1995). Changes in NADP linked isocitrate dehydrogenase during tomato fruit ripening. Characterization of the predominant cytosolic enzyme from green and ripe pericarp. 10.1007/BF00193228
Cantón FR, García-Gutiérrez A, Cánovas FM(1995)Molecular cloning and expression of a pine cDNA encoding glutamine synthetase. Investigación Agraria, Sistemas y recursos forestales, Fuera de serie nº4, 169-171.
Pérez-García A, Cánovas FM, Hirel B, Gallardo F, de Vicente A (1995)Differential expression of glutamine synthetase isoforms in tomato detached leaflets infected with Pseudomonas syringae pv. tomato Molecular Plant Microbe Interactions, 8, 96-103.
Cantón FR, García-Gutiérrez A, Gallardo F, de Vicente A, Cánovas FM. (1993). Molecular characterization of a cDNA clone encoding glutamine synthetase from a gymnosperm: Pinus sylvestris. Plant Mol. Biol., 22, 819-828.
Gallardo F, Cantón FR, Garcia-Gutierrez A, Cánovas FM (1993). Changes in photorespiratory enzymes and glutamate synthases in ripening tomatoes. Plant Physiol Biochem, 31, 189-196.
García-Gutiérrez A, Cantón FR, Gallardo F, Cánovas FM (1993). Immunochemical analysis of chloroplast polypeptides from maritime pine. Phytochemistry, 31, 337-341
Gallardo F, Cánovas F (1992). A macromolecular inhibitor of glutamine synthetase activity in tomato root extracts. Phytochemistry, 31, 2267-2271.
Cánovas FM, Cantón FR, Gallardo F, García-Gutiérrez A, de Vicente A (1991). Accumulation of glutamine synthetase during early development of maritime pine (Pinus pinaster) seddling s. Planta 185, 372-378.
